Recombinant Human MMP-9

Pricing & Details

Product. No.M1254
Alternate Names
CLG4B, Gelatinase B (GELB)
Expression Host
CHO
Reactivity Species
Human
Prod No.
Size
Price
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Qty
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M1254-10 µg
10 µg
$389.00
In stock
Max:
Min: 1
Step: 1

Background

Matrix metallopeptidase 9, also known as MMP9, is a biological enzyme that is a member of the matrix metalloproteinases (MMPs). MMP9 is thought to play a critical role in tumor cell invasion,1 breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-9 is secreted from cells and, once activated, is thought to degrade collagen in the extracellular matrix.2 MMP-9 is one of the major proteases produced by osteoclasts and possibly plays a role in osteoclastic bone resorption.3

Protein Details

Purity
>90% by SDS Page and analyzed by silver stain.
Endotoxin Level
< 1.1 EU/μg as determined by the LAL method
Biological Activity
The biological activity of Human MMP-9 was determined by its ability to cleave a fluorescent peptide substrate, (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Ala-Arg-NH2. The specific activity, measured under the described conditions, is > 1,300 pmoles/min/μg.
Expression Host
CHO
Protein Accession No.
Amino Acid Sequence
a prqrqstlvl fpgdlrtnlt drqlaeeyly rygytrvaem rgeskslgpa llllqkqlsl petgeldsat lkamrtprcg vpdlgrfqtf egdlkwhhhn itywiqnyse dlpravidda farafalwsa vtpltftrvy srdadiviqf gvaehgdgyp fdgkdgllah afppgpgiqg dahfdddelw slgkgvvvpt rfgnadgaac hfpfifegrs ysacttdgrs dglpwcstta nydtddrfgf cpserlytqd gnadgkpcqf pfifqgqsys acttdgrsdg yrwcattany drdklfgfcp tradstvmgg nsagelcvfp ftflgkeyst ctsegrgdgr lwcattsnfd sdkkwgfcpd qgyslflvaa hefghalgld hssvpealmy pmyrftegpp lhkddvngir hlygprpepe prppttttpq ptapptvcpt gpptvhpser ptagptgpps agptgpptag pstattvpls pvddacnvni fdaiaeignq lylfkdgkyw rfsegrgsrp qgpfliadkw palprkldsv feerlskklf ffsgrqvwvy tgasvlgprr ldklglgadv aqvtgalrsg rgkmllfsgr rlwrfdvkaq mvdprsasev drmfpgvpld thdvfqyrek ayfcqdrfyw rvssrselnq vdqvgyvtyd ilqcped
N-terminal Sequence Analysis
Ala20
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human MMP-9 is Mr 77 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 93 kDa.
Formulation
The protein is 0.2 μm filtered and provided as a solution in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5.
Country of Origin
USA
Shipping
Next Day 2-8°C
PubMed
NCBI Gene Bank

References & Citations

1. Viswanadha, VP. et al. (2017) J Cell Biochem. 118(9):2865-2876.
2. Mobashery, S. et al. (1995) Cancer Res. 55: 2548
3. Tökés, ZA.et al. (1995) J Neurochem. 64: 1312
4. Kumegawa, M. et al. (1994) J Biol. Chem. 269: 15006
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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