Anti-Mouse MMP-9
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen NS0-Derived Recombinant Mouse MMP-9 Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831297 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse MMP-9 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MMP-9 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Immunocytochemistry: This antibody will detect MMP-9 in cells or tissues. The working dilution is 2-15 μg/mL. Additional Applications Reported In Literature ? Immunoprecipitation: Suitable for use at concentration of 25 µg/mL. Immunohistochemistry: Suitable for at concentration of 5-15 µg/mL. Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Matrix Metalloproteinase 9 (MMP-9) recognizes Mouse MMP-9. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Matrix metallopeptidase 9, also known as MMP9, is a biological enzyme that is a member of the matrix metalloproteinases (MMPs). MMP9 is thought to play a critical role in tumor cell invasion,1 breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-9 is secreted from cells and, once activated, is thought to degrade collagen in the extracellular matrix.2 MMP-9 is one of the major proteases produced by osteoclasts and possibly plays a role in osteoclastic bone resorption.3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Viswanadha, VP. et al. (2017) J Cell Biochem. 118(9):2865-2876. 2. Mobashery, S. et al. (1995) Cancer Res. 55: 2548 3. Tökés, ZA.et al. (1995) J Neurochem. 64: 1312 4. Kumegawa, M. et al. (1994) J Biol. Chem. 269: 15006 Technical ProtocolsICC    |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
