Anti-Mouse MMP-9 – Biotin

Pricing & Details

Product No.M1262
Protein
MMP-9
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
CLG4B, Gelatinase B (GELB)
Applications
ICC
,
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
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M1262-50 µg
50 µg
$419.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse MMP-9 (Accession # P41245)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse MMP-9 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MMP-9 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M1253.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:A106. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse matrix metalloproteinase 9 (MMP-9) recognizes Mouse MMP-9. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Matrix metallopeptidase 9, also known as MMP9, is a biological enzyme that is a member of the matrix metalloproteinases (MMPs). MMP9 is thought to play a critical role in tumor cell invasion,1 breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-9 is secreted from cells and, once activated, is thought to degrade collagen in the extracellular matrix.2 MMP-9 is one of the major proteases produced by osteoclasts and possibly plays a role in osteoclastic bone resorption.3

Antigen Details

Protein
PubMed

References & Citations

1. Viswanadha, VP. et al. (2017) J Cell Biochem. 118(9):2865-2876.
2. Mobashery, S. et al. (1995) Cancer Res. 55: 2548
3. Tökés, ZA.et al. (1995) J Neurochem. 64: 1312
4. Kumegawa, M. et al. (1994) J Biol. Chem. 269: 15006
IHC
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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