Recombinant Mouse MMP-9

Recombinant Mouse MMP-9

Product No.: M1253

[product_table name="All Top" skus="M1253"]

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Alternate Names
CLG4B, Gelatinase B (GELB)
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Mouse

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Background

Matrix metallopeptidase 9, also known as MMP9, is a biological enzyme that is a member of the matrix metalloproteinases (MMPs). MMP9 is thought to play a critical role in tumor cell invasion,1 breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-9 is secreted from cells and, once activated, is thought to degrade collagen in the extracellular matrix.2 MMP-9 is one of the major proteases produced by osteoclasts and possibly plays a role in osteoclastic bone resorption.3

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The specific activity, measured with 10 μM ES001 and 20 ng enzyme in 100 μL of TCNB (50 mM Tris-HCl, pH 7.5, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij 35) at room temperature, is > 1,500 pmoles/min/μg.
Protein Accession No.
Amino Acid Sequence
a pyqrqptfvv fpkdlktsnl tdtqlaeayl yrygytraaq mmgekqslrp allmlqkqls lpqtgeldsq tlkairtprc gvpdvgrfqt fkglkwdhhn itywiqnyse dlprdmidda farafavwge vapltftrvy gpeadiviqf gvaehgdgyp fdgkdgllah afppgagvqg dahfdddelw slgkgvvipt yygnsngapc hfpftfegrs ysacttdgrn dgtpwcstta dydkdgkfgf cpserlyteh gngegkpcvf pfifegrsys acttkgrsdg yrwcattany dqdklygfcp trvdatvvgg nsagelcvfp fvflgkqyss ctsdgrrdgr lwcattsnfd tdkkwgfcpd qgyslflvaa hefghalgld hssvpealmy plysylegfp lnkddidgiq ylygrgskpd prppatttte pqptapptmc ptipptaypt vgptvgptga pspgptssps pgptgapspg ptaaptagss easteslspa dnpcnvdvfd aiaeiqgalh ffkdgwywkf lnhrgsplqg pfltartwpa lpatldsafe dpqtkrvfff sgrqmwvytg ktvlgprsld klglgpevth vsgllprrpg kallfskgrv wrfdlksqkv dpqsvirvdk efsgvpwnsh difqyqdkay fchgkffwrv sfqnevnkvd pevnqvddvg yvtydllqcp
N-terminal Sequence Analysis
Ala20
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse MMP-9 is Mr 78 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 80-105 kDa.
Predicted Molecular Mass
78
Formulation
Supplied as a 0.2 μm filtered solution of TCNB (50 mM Tris-HCl, pH 7.5, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij 35) at a concentration of 0.2 mg/ml.
Storage and Stability
This protein is stable for one month when stored sterile at -20°C or for three months when stored at -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
NCBI Gene Bank

References & Citations

1. Viswanadha, VP. et al. (2017) J Cell Biochem. 118(9):2865-2876.
2. Mobashery, S. et al. (1995) Cancer Res. 55: 2548
3. Tökés, ZA.et al. (1995) J Neurochem. 64: 1312
4. Kumegawa, M. et al. (1994) J Biol. Chem. 269: 15006
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.