Recombinant Mouse MMP-9

Matrix metallopeptidase 9, also known as MMP9, is a biological enzyme that is a member of the matrix metalloproteinases (MMPs). MMP9 is thought to play a critical role in tumor cell invasion,¹ breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-9 is secreted from cells and, once activated, is thought to degrade collagen in the extracellular matrix.² MMP-9 is one of the major proteases produced by osteoclasts and possibly plays a role in osteoclastic bone resorption.³

The specific activity, measured with 10 μM ES001 and 20 ng enzyme in 100 μL of TCNB (50 mM Tris-HCl, pH 7.5, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij 35) at room temperature, is > 1,500 pmoles/min/μg.

a pyqrqptfvv fpkdlktsnl tdtqlaeayl yrygytraaq mmgekqslrp allmlqkqls lpqtgeldsq tlkairtprc gvpdvgrfqt fkglkwdhhn itywiqnyse dlprdmidda farafavwge vapltftrvy gpeadiviqf gvaehgdgyp fdgkdgllah afppgagvqg dahfdddelw slgkgvvipt yygnsngapc hfpftfegrs ysacttdgrn dgtpwcstta dydkdgkfgf cpserlyteh gngegkpcvf pfifegrsys acttkgrsdg yrwcattany dqdklygfcp trvdatvvgg nsagelcvfp fvflgkqyss ctsdgrrdgr lwcattsnfd tdkkwgfcpd qgyslflvaa hefghalgld hssvpealmy plysylegfp lnkddidgiq ylygrgskpd prppatttte pqptapptmc ptipptaypt vgptvgptga pspgptssps pgptgapspg ptaaptagss easteslspa dnpcnvdvfd aiaeiqgalh ffkdgwywkf lnhrgsplqg pfltartwpa lpatldsafe dpqtkrvfff sgrqmwvytg ktvlgprsld klglgpevth vsgllprrpg kallfskgrv wrfdlksqkv dpqsvirvdk efsgvpwnsh difqyqdkay fchgkffwrv sfqnevnkvd pevnqvddvg yvtydllqcp

Ala20

Solution

The predicted molecular weight of Recombinant Mouse MMP-9 is Mr 78 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 80-105 kDa.

78

Supplied as a 0.2 μm filtered solution of TCNB (50 mM Tris-HCl, pH 7.5, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij 35) at a concentration of 0.2 mg/ml.

This protein is stable for one month when stored sterile at -20°C or for three months when stored at -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

1. Viswanadha, VP. et al. (2017) J Cell Biochem. 118(9):2865-2876.
2. Mobashery, S. et al. (1995) Cancer Res. 55: 2548
3. Tökés, ZA.et al. (1995) J Neurochem. 64: 1312
4. Kumegawa, M. et al. (1994) J Biol. Chem. 269: 15006