Recombinant Human PD-L1

Recombinant Human PD-L1

Product No.: P614

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Alternate Names
PDL1, CD274
Expression Host
HEK-293 Cells
Applications
ELISA
FA
SDS-PAGE
WB

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Select Product Size
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Background

Human Programmed Death-Ligand 1 (PD-L1) is a protein that is a part of the immune checkpoint pathway which regulates the system used to balance the interaction between tumor cells and immune surveillance 1. Unlike PD-1, which is mainly found on T-cells and acts as a receptor, PD-L1 is predominantly present on tumor cells and antigen-presenting cells. It serves as a mediator of immune tolerance by exploiting natural pathways that prevent autoimmunity. Tumors can evade the immune system and avoid detection by expressing PD-L1, inhibiting T-cell activation 2 . Inflammatory cytokines in the tumor microenvironment further enhance this evasion strategy by increasing PD-L1 expression 3 . This highlights how tumors adapt and utilize the body's checkpoints for survival and growth. The complex involvement of PD-L1 in both tolerance and cancer immunoevasion emphasizes its significance as a target, for therapy. Blocking PD-L1 aims to dismantle the shield it provides to tumors allowing the immune system to regain its ability to eliminate cells 4 .

Protein Details

Species
Human
Format
Purified No Carrier Protein
Protein Accession No.
Q15116
Amino Acid Sequence
FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERT
State of Matter
Lyophilized
SDS-Page Molecular Weight
~40k Reduced Conditions
Predicted Molecular Mass
~25k
Reconstitution
Reconstitute at 0.1-1 mg/ml using filtered deionized water. Gently mix by vortexing and/or inversion until fully dissolved. Centrifuge if necessary.
Storage and Stability
This lyophilized protein is stable for twelve months when stored at -20°C to -70°C. After aseptic reconstitution, this protein may be stored for one month at 2°C to 8°C or for three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Frozen Dry Ice
Ligand/Receptor
PD1
Species
Human
Regulatory Status
Research Use Only
NCBI Gene Bank
UniProt.org
Applications and Recommended Usage ?
(Quality Tested by Leinco)
SDS-PAGE,
WB,
ELISA,
FA

References & Citations

1. Yi M, Niu M, Xu L, Luo S, Wu K. J Hematol Oncol. 2021;14(1):10.
2. McDermott DF, Atkins MB. Cancer Medicine. 2013;2(5):662-673.
3. Dermani FK, Samadi P, Rahmani G, Kohlan AK, Najafi R. Journal of Cellular Physiology. 2019;234(2):1313-1325.
4. Dong Y, Sun Q, Zhang X. Oncotarget. 2017;8(2):2171-2186.
Indirect ELISA Protocol
FA
SDS-PAGE
General Western Blot Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.