TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain,” which activates the cell's apoptotic machinery. Recombinant human soluble TRAIL/Apo2L is a 168 amino acid polypeptide (19.6 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL/Apo2L protein.
>97% by SDS-PAGE and HPLC
<0.1 EU/µg as determined by the LAL method
The biological activity of Human TNF-Related Apoptosis Inducing Ligand/Apo2 Ligand is determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Protein Accession No.
Amino Acid Sequence
vrergpqrva ahitgtrgrs ntlsspnskn ekalgrkins wessrsghsf lsnlhlrnge lvihekgfyy iysqtyfrfq eeikentknd kqmvqyiyky tsypdpillm ksarnscwsk daeyglysiy qggifelken drifvsvtne hlidmdheas ffgaflvg
N-terminal Sequence Analysis
Val114 (with and without an N-terminal Met)
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human TRAIL/Apo2L is Mr 19 kDa.
Lyophilized from a 0.2 μm filtered solution in Tris, (NH4)2SO4 and ZnSO4.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
References & Citations
1. Rehm, M. et al. (2008) Biochim Biophys Acta. 1783(10):1903-13. Article Link
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.