Recombinant Human TRAIL/Apo2L

Pricing & Details

Product. No.T164
Alternate Names
Apo2 Ligand, TNFSF10
Expression Host
E. coli
Reactivity Species
Human
Prod No.
Size
Price
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T164-10 µg
10 µg
$339.00
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Min: 1
Step: 1

Background

TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain,” which activates the cell's apoptotic machinery. Recombinant human soluble TRAIL/Apo2L is a 168 amino acid polypeptide (19.6 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL/Apo2L protein.

Protein Details

Purity
>97% by SDS-PAGE and HPLC
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human TNF-Related Apoptosis Inducing Ligand/Apo2 Ligand is determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Expression Host
E. coli
Protein Accession No.
Q6IBA9
Amino Acid Sequence
vrergpqrva ahitgtrgrs ntlsspnskn ekalgrkins wessrsghsf lsnlhlrnge lvihekgfyy iysqtyfrfq eeikentknd kqmvqyiyky tsypdpillm ksarnscwsk daeyglysiy qggifelken drifvsvtne hlidmdheas ffgaflvg
N-terminal Sequence Analysis
Val114 (with and without an N-terminal Met)
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human TRAIL/Apo2L is Mr 19 kDa.
Formulation
Lyophilized from a 0.2 μm filtered solution in Tris, (NH4)2SO4 and ZnSO4.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed

References & Citations

1. Rehm, M. et al. (2008) Biochim Biophys Acta. 1783(10):1903-13. Article Link
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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