Anti-Human TRAIL (Clone 75411)

Purified Recombinant Human TRAIL (Accession # P50591)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

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IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human TRAIL bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human TRAIL (at 12 ng/ml) is 2-8 µg/ml.

Mouse Anti-Human TNF-Related Apoptosis-Inducing Ligand (TRAIL) (Clone 75411) recognizes an epitope on Human TRAIL. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

TNF-related apoptosis-inducing ligand (TRAIL) belongs to the tumor necrosis factor family and activates rapid apoptosis in tumor cells, and binds to the death-signaling receptor DR4.¹ TRAIL is a potential tumor specific cancer therapeutic agent, since it selectively induces apoptosis in transformed versus normal cells.²

1. Ashkenazi, A. et al. (1997) Science 227: 818
2. Romeo, G. et al. (2005) Current Pharmaceutical Design 11: 933