Recombinant Human TWEAK R

TWEAK R (TNFRSF12A/FN14) belongs to the TNF family of transmembrane proteins. The receptor contains a binding site for members of the TNFR-associated factor (TRAF) family that promotes the activation of NF-κB. It is expressed in many tissues such as heart, placenta, kidney, pancreas, skeletal muscle and lung and is regulated by a number of different growth factors (1). TWEAK R plays a role in TWEAK-induced endothelial cell migration, proliferation and angiogenesis (2). TWEAK-induced cell death via TWEAK R includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. TWEAK R is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs (3). Recent studies have suggested some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity and cancer treatment (4).

The biological activity of Human TNF-Related Weak Inducer of Apoptosis Receptor is determined by its ability to inhibit TWEAK-induced weak cell death of HT29 cells. The expected ED₅₀ for this effect is 1.0-3.0 µg/ml.

Fc Fusion Protein

eqa pgtapcsrgs swsadldkcm dcascrarph sdfclgcaaa ppapfrllwi egrmdpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwqqgn vfscsvmhea lhnhytqksl slspgk

Glu28

Lyophilized

The predicted molecular weight of Recombinant Human TNF-Related Weak Inducer of Apoptosis Receptor (TWEAK R) is Mr 32 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 35-45 kDa.

32

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

1. Nakayama, M. et al. (2003) J. Immunol. 170:341
2. Wiley, SR. et al. (2001) Immunity 15:837
3. Feng, SL. et al. (2000) Am. J. Pathol. 156:1253
4. Desplat-Jégo, S. et al. (2005) Clin. Immunol. 117:15