Recombinant Mouse Flt-3 Ligand

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Pricing & Details

Prod. No.F159
Expression Host
NS0 cells
Size
Price
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5 µg
159.00
Max:
Min: 1
Step: 1
25 µg
499.00
Max:
Min: 1
Step: 1

Data

Background

Fms-related tyrosine kinase 3 ligand (FLT3LG) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, such as IL-7 and IL-3, Flt-3 ligand promotes long-term expansion and differentiation of human pro-B-cells.1,2 Flt3 ligand is a cytokine capable of inducing large numbers of dendritic cells that dramatically enhances the sensitivity of antigen-specific B and T cell responses to systemic injection of a soluble protein. Through a CD40-CD40 ligand-dependent mechanism Flt-3 Ligand influences the class of antibody produced and enables productive immune responses.3

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse FLT3LG was determined in a cell proliferation assay. The expected ED50=1-4ng/ml
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
gtpd cyfshspiss nfkvkfrelt dhllkdypvt vavnlqdekh ckalwslfla qrwieqlktv agskmqtlle dvnteihfvt sctfqplpec lrfvqtnish llkdtctqll alkpcigkac qnfsrclevq cqpdsstllp prspialeat elpeprpr
N-terminal Sequence Analysis
Gly27
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse Flt-3 Ligand is Mr 18 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 18-32 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 45% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Mattson, J. et al. (1994) Nature 368: 643
2. Cleveland, LS. et al. (1995) Oncogene 10: 149
3. Maliszewski, CR. et al. (1998) J. Exp. Med. 188: 2075
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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