Anti-Human Flt-3 Ligand (Clone 40406)

Pricing & Details

Product No.F1071
Clone
40406
Protein
Flt-3 Ligand
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fms-Related Tyrosine Kinase 3 Ligand, Flk-2 Ligand, FL, FLT3LG, STK-1 Ligand
Isotype
Mouse
IgG1
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
F1071-25 µg
25 µg
$165.00
In stock
Max:
Min: 1
Step: 1
F1071-100 µg
100 µg
$430.00
In stock
Max:
Min: 1
Step: 1
F1071-500 µg
500 µg
$935.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human Flt-3 Ligand (Accession # AAA17999)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human Flt-3 Ligand this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Flt-3 Ligand is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F1031.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human Flt-3 Ligand bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human Flt-3 Ligand (at 5.0 ng/ml) is 0.02 - 0.06 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Flt-3 Ligand (Clone 40406) recognizes an epitope on Human Flt-3 Ligand. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Fms-related tyrosine kinase 3 ligand (FLT3LG) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, such as IL-7 and IL-3, Flt-3 ligand promotes long-term expansion and differentiation of human pro-B-cells.1,2 Flt3 ligand is a cytokine capable of inducing large numbers of dendritic cells that dramatically enhances the sensitivity of antigen-specific B and T cell responses to systemic injection of a soluble protein. Through a CD40-CD40 ligand-dependent mechanism Flt-3 Ligand influences the class of antibody produced and enables productive immune responses.3

Antigen Details

References & Citations

1. Mattson, J. et al. (1994) Nature 368: 643
2. Cleveland, LS. et al. (1995) Oncogene 10: 149
3. Maliszewski, CR. et al. (1998) J. Exp. Med. 188: 2075
General Western Blot Protocol

Formats Available

Prod No.
Description
F1071
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Leinco Technologies uses cookies to improve your experience and our website service. By continuing to browse our website, you accept our cookie policy. Ok