Anti-Mouse Flt-3 Ligand

Anti-Mouse Flt-3 Ligand

Product No.: F1062

[product_table name="All Top" skus="F1062"]

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Target
Flt-3 Ligand
Product Type
Polyclonal Antibody
Alternate Names
Fms-Related Tyrosine Kinase 3 Ligand, Flk-2 Ligand, FL, FLT3LG, STK-1 Ligand
Applications
ELISA Cap
,
ICC
,
N
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse Flt-3 Ligand (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/mL . A suitable detection antibody is PN:F1096 at a concentration of approximately 200 ng/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is ~1.0 ng/well. A suggested standard for this assay is PN:F159.
Western Blotting: To detect Mouse Flt-3 Ligand this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Flt-3 Ligand is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F159.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse Flt-3 Ligand bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse Flt-3 Ligand (at 10 ng/ml) is 0.05-0.25 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Flt-3 Ligand recognizes Mouse Flt-3 Ligand. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Fms-related tyrosine kinase 3 ligand (FLT3LG) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, such as IL-7 and IL-3, Flt-3 ligand promotes long-term expansion and differentiation of human pro-B-cells.1,2 Flt3 ligand is a cytokine capable of inducing large numbers of dendritic cells that dramatically enhances the sensitivity of antigen-specific B and T cell responses to systemic injection of a soluble protein. Through a CD40-CD40 ligand-dependent mechanism Flt-3 Ligand influences the class of antibody produced and enables productive immune responses.3

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Mattson, J. et al. (1994) Nature 368: 643
2. Cleveland, LS. et al. (1995) Oncogene 10: 149
3. Maliszewski, CR. et al. (1998) J. Exp. Med. 188: 2075
Elisa Sandwich Protocol
ICC
N
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.