Recombinant Human Fms-related tyrosine kinase 3 ligand (FLT3LG) Ligand

Fms-related tyrosine kinase 3 ligand (FLT3LG) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, such as IL-7 and IL-3, Flt-3 ligand promotes long-term expansion and differentiation of human pro-B-cells.^1,2 Flt3 ligand is a cytokine capable of inducing large numbers of dendritic cells that dramatically enhances the sensitivity of antigen-specific B and T cell responses to systemic injection of a soluble protein. Through a CD40-CD40 ligand-dependent mechanism Flt-3 Ligand influences the class of antibody produced and enables productive immune responses.³

The biological activity of Human flt3-Ligand was determined by its ability to stimulate the proliferation of a mouse Flt3 transfected BaF/3 cell line. The expected ED₅₀ for this effect is typically 0.2 - 1 ng/ml.

AAA17999.1

tqdc sfqhspissd favkirelsd yllqdypvtv asnlqdeelc galwrlvlaq rwmerlktva gskmqgller vnteihfvtk cafqpppscl rfvqtnisrl lqetseqlva lkpwitrqnf srclelqcqp dsstlpppws prpleatapt apqpp

Thr27

Lyophilized

The predicted molecular weight of Recombinant Human FLT3LG is Mr 17.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 17-30 kDa (reducing conditions).

17.5

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Mattson, J. et al. (1994) Nature 368: 643
2. Cleveland, LS. et al. (1995) Oncogene 10: 149
3. Maliszewski, CR. et al. (1998) J. Exp. Med. 188: 2075