Recombinant Mouse IL-17A/F
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Protein DetailsPurity >95% by SDS Page and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Protein Accession No. Amino Acid Sequence ( Mouse IL-17 A) tvkaaaiipq ssacpnteak dflqnvkvnl kvfnslgakv ssrrpsdyln rstspwtlhr nedpdrypsv iweaqcrhqr cvnaegkldh hmnsvliqqe ilvlkrepes cpftfrvekm lvgvgctcva sivrqaa, (Mouse IL-17F) rknpkagvpa lqkagncppl edntvrvdir ifnqnqgisv prefqnrsss pwdynitrdp hrfpseiaea qcrhsgcina qgqedstmns vaiqqeilvl rrepqgcsns frlekmllkv gctcvkpivh qaa N-terminal Sequence Analysis Thr22 (IL-17A) & Met (IL-17F) State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Mouse IL17A/ F is Mr IL-17A: 14.9; IL-17F: 15.5 kDa. Predicted Molecular Mass IL-17A: 14.9; IL-17F: 15.5 Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient Applications and Recommended Usage ? (Quality Tested by Leinco) ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. Flow Cytometry: PN:A106 Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS. Technical ProtocolsCertificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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