Recombinant Mouse IL-17A/F

Recombinant Mouse IL-17A/F

Product No.: I-847

[product_table name="All Top" skus="I-847"]

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Alternate Names
Interleukin-17A/F Heterodimer
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Mouse
Applications
ELISA Cap
FC

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Select Product Size
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Protein Details

Purity
>95% by SDS Page and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Amino Acid Sequence
( Mouse IL-17 A) tvkaaaiipq ssacpnteak dflqnvkvnl kvfnslgakv ssrrpsdyln rstspwtlhr nedpdrypsv iweaqcrhqr cvnaegkldh hmnsvliqqe ilvlkrepes cpftfrvekm lvgvgctcva sivrqaa, (Mouse IL-17F) rknpkagvpa lqkagncppl edntvrvdir ifnqnqgisv prefqnrsss pwdynitrdp hrfpseiaea qcrhsgcina qgqedstmns vaiqqeilvl rrepqgcsns frlekmllkv gctcvkpivh qaa
N-terminal Sequence Analysis
Thr22 (IL-17A) & Met (IL-17F)
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IL17A/ F is Mr IL-17A: 14.9; IL-17F: 15.5 kDa.
Predicted Molecular Mass
IL-17A: 14.9; IL-17F: 15.5
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.
Elisa Sandwich Protocol
Flow Cytometry
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Prod No.
Description
I-847
Products are for research use only. Not for use in diagnostic or therapeutic procedures.