Recombinant Mouse IL-3 Rα

IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL-3 R beta chain has a very large cytoplasmic domain. Recent studies suggest that formation of IL-3 R beta-beta homodimers initiates signaling events. The IL-3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes.

The typical ED₅₀ is 0.3 - 0.15 µg/ml in the presence of 2.5 µg/ml of rMouse IL-3 beta (PN:I-568) and 2 ng/ml of PN:I-206.

Fc Fusion Protein

sdlaavreap ptavttpiqn lhidpahytl swdpapgadi ttgafcrkgr difvwadpgl arcsfqslsl chvtnftvfl gkdravagsi qfppdddgdh eaaaqdlrcw vhegqlscqw ergpkatgdv hyrmfwrdvr lgpahnrecp hyhsldvnta gpaphggheg ctldldtvlg stpnspdlvp qvtitvngsg ragpvpcmdn tvdlqraevl apptltvecn gseaharwva rnrfhhgllg ytlqvnqssr sepqeynvsi phfwvpnaga isfrvksrse vyprklssws eawglvcppe vmpvkkscdk thtcppcpap ellggpsvfl fppkpkdtlm isrtpevtcv vvdvshedpe vkfnwyvdgv evhnaktkpr eeqynstyrv vsvltvlhqd wlngkeykck vsnkalpapi ektiskakgq prepqvytlp psrdeltknq vsltclvkgf ypsdiavewe sngqpennyk ttppvldsdg sfflyskltv dksrwqqgnv fscsvmheal hnhytqksls lspgk

Ser17

Lyophilized

The predicted molecular weight of Recombinant Mouse IL-3 Rα is Mr 61.0 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 70-80 kDa.

61.0

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Garnache-Ottou, F. et al. (2015) Haematologica. 100(2):223-30
2. Phillips, GL. et al. (2000) Leukemia 14: 1777