Anti-Human IL-3 Rα
Pricing & Details
Purified Recombinant Human IL-3 Rα (Accession # P26951)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-3 Rα. A suggested method would be to stain cells expressing IL-3 Rα with 2.5 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:M1189.
Western Blotting: To detect Human IL-3 Rα this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-3 Rα is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-559.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 - 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human IL-3 Rα bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-3 Rα (at 0.5 ng/ml) is 0.6 - 1.2 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
mmunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Interleukin 3 Soluble Receptor Alpha (IL-3 Rα) (Clone 32703) recognizes an epitope on Human IL-3 Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a type I transmembrane protein that is expressed on the large majority of progenitor cells. IL-3R alpha function can be defined by the activity of its ligand interleukin-3. IL-3 is a multipotent cytokine that promotes the development of hemopoietic progenitors into cells of the erythroid, myeloid and lymphoid lineages.1 CD123 may be a promising strategy for the preferential ablation of acute myelogenous leukemia (AML) cells.2
References & Citations
1. Garnache-Ottou, F. et al. (2015) Haematologica. 100(2):223-30
2. Phillips, GL. et al. (2000) Leukemia 14: 1777
Products are for research use only. Not for use in diagnostic or therapeutic procedures.