Anti-Human IL-3 Rα (CD123)
Anti-Human IL-3 Rα (CD123)
Product No.: I-651
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Clone 32703 Target IL-3 R alpha Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-3 Soluble Receptor Alpha, IL3RA, CD123, IL3R, IL3RAY, IL3RX, IL3RY, MGC34174, hIL-3Ra Isotype IgG1 Applications CyTOF® , FC , ICC , IHC FFPE , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IL-3 Rα (Accession # P26951) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830773 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-3 Rα. A suggested method would be to stain cells expressing IL-3 Rα with 2.5 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:M1189. Western Blotting: To detect Human IL-3 Rα this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-3 Rα is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-559. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 - 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human IL-3 Rα bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-3 Rα (at 0.5 ng/ml) is 0.6 - 1.2 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. mmunocytochemistry: Suitable for use at concentration of 8-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Interleukin 3 Soluble Receptor Alpha (IL-3 Rα) (Clone 32703) recognizes an epitope on Human IL-3 Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a type I transmembrane protein that is expressed on the large majority of progenitor cells. IL-3R alpha function can be defined by the activity of its ligand interleukin-3. IL-3 is a multipotent cytokine that promotes the development of hemopoietic progenitors into cells of the erythroid, myeloid and lymphoid lineages.1 CD123 may be a promising strategy for the preferential ablation of acute myelogenous leukemia (AML) cells.2 Antigen DetailsPubMed References & Citations1. Garnache-Ottou, F. et al. (2015) Haematologica. 100(2):223-30 2. Phillips, GL. et al. (2000) Leukemia 14: 1777 Technical ProtocolsCyTOF®  ICC IHC FFPE N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
