Osteopontin (OPN) also known as bone sialoprotein I (BSP-1), early T-lymphocyte activation (ETA-1), and Secreted phosphoprotein 1 (SPP1) is a secreted phosphoprotein that confers on cancer cells a migratory phenotype and activates signaling pathways that induce cell survival, proliferation, invasion, and metastasis.1 OPN is also considered as a proinflammatory cytokine.2 OPN has been studied as a multifunctional protein that is upregulated in a variety of acute and chronic inflammatory conditions, such as wound healing, fibrosis, autoimmune disease, and atherosclerosis. OPN is highly expressed at sites with atherosclerotic plaques, especially those associated with macrophages and foam cells.3
>95% by SDS-PAGE and analyzed by silver stain.
<0.01EU/µg as determined by the LAL method
Fusion Protein Tag
Protein Accession No.
Amino Acid Sequence
lpvk vtdsgsseek lyslhpdpia twlvpdpsqk qnllapqnav sseekddfkq etlpsnsnes hdhmdddddd ddddgdhaes edsvdsdesd eshhsdesde tvtastqadt ftpivptvdv pngrgdslay glrsksrsfq vsdeqypdat dedltshmks geskesldvi pvaqllsmps dqdnngkgsh essqldepsl ethrlehske sqesadqsdv idsqasskas lehqshkfhs hkdklvldpk skeddrylkf rishelesss sevnhhhhhh
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse Osteopontin is Mr 31.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 65 dKa doublet and a 30 kDa band.
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Arafat, H. et al. (2009) Surgery. 146(2):232-40.
2. Xue, S. et al. (2009) Heart Vessels 24: 116
3. Kim, HS. et al. (2009) Curr Atheroscler Rep. 11: 206
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.