Purified Recombinant Mouse OPN (Accession # Q547B5)
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse OPN this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse OPN is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:O121.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:O131 as the capture antibody at 0.2 - 0.8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse OPN.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Mouse Osteopontin (OPN) recognizes Mouse OPN. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Osteopontin (OPN) also known as bone sialoprotein I (BSP-1), early T-lymphocyte activation (ETA-1), and Secreted phosphoprotein 1 (SPP1) is a secreted phosphoprotein that confers on cancer cells a migratory phenotype and activates signaling pathways that induce cell survival, proliferation, invasion, and metastasis.1 OPN is also considered as a proinflammatory cytokine.2 OPN has been studied as a multifunctional protein that is upregulated in a variety of acute and chronic inflammatory conditions, such as wound healing, fibrosis, autoimmune disease, and atherosclerosis. OPN is highly expressed at sites with atherosclerotic plaques, especially those associated with macrophages and foam cells.3
NCBI Gene Bank ID
References & Citations
1. Arafat, H. et al. (2009) Int J Cancer 11(5):373-381.
2. Xue, S. et al. (2009) Heart Vessels 24: 116
3. Kim, HS. et al. (2009) Curr Atheroscler Rep. 11: 206
Products are for research use only. Not for use in diagnostic or therapeutic procedures.