Recombinant Mouse Thrombopoietin (NS0 Expressed)

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Pricing & Details

Product. No.T220
Alternate Names
THPO, MGC163194, Megakaryocyte Growth and Development Factor (MGDF), MKCSF, ML, MPLLG, MpI Ligand, Leukemia Virus Oncogene Ligand
Expression Host
NS0 cells
Species
Mouse
Prod No.
Size
Price
Avail.
Qty
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T220-5 µg
5 µg
$329.00
In stock
Max:
Min: 1
Step: 1
T220-25 µg
25 µg
$895.00
In stock
Max:
Min: 1
Step: 1
T220-200 ml
200 ml
$1,299.00
In stock
Max:
Min: 1
Step: 1

Data

Background

Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.1 TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis2 and platelet production.1

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse TPO was determined by a cell proliferation assay using MO7e cells (Avanzi, G. et al., 1988, Br. J. Haematol. 69:359). The expected ED50 for this effect is typically 1 - 3 ng/ml.
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
sspvapacdp rllnkllrds hllhsrlsqc pdvdplsipv llpavdfslg ewktqteqsk aqdilgavsl llegvmaarg qlepsclssl lgqlsgqvrl llgalqgllg tqlplqgrtt ahkdpnalfl slqqllrgkv rflllvegpt lcvrrtlptt avpsstsqll tlnkfpnrts glletnfsvt artagpglls rlqgfrvkit pgqlnqtsrs pvqisgylnr thgpvngthg lfagtslqtl easdispgaf nkgslafnlq gglppspsla pdghtpfpps palptthgsp pqlhplfpdp sttmpnstap hpvtmyphpr nlsqet
N-terminal Sequence Analysis
Ser22
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse TPO is Mr 35 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 80-90 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Vainchenker, W. et al. (1998) Eur Cytokine Newt. 9: 221
2. Skoda, RC. et al. (1998) Blood 92: 4023
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.