Recombinant Mouse VEGF 164

Recombinant Mouse VEGF 164

Product No.: V102

[product_table name="All Top" skus="V102"]

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Alternate Names
Vascular Endothelial Growth Factor 164, VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen
Product Type
Recombinant Protein
Expression Host
sf Insect Cells
Species
Mouse

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Product Size
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Background

Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse VEGF 164 was determined in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The expected ED<sub>50</sub> is 2-4 ng/ml.
Protein Accession No.
Amino Acid Sequence
aptt egeqkshevi kfmdvyqrsy crpietlvdi fqeypdeiey ifkpscvplm rcagccndea lecvptsesn itmqimrikp hqsqhierms flqhsrcecr pkkdrtkpen hcepcserrk hlfvqdpqtc kcsckntdsr ckarqlelne rtcrcdkprr
N-terminal Sequence Analysis
Ala27
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse VEGF 164 is Mr 19.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 42 kDa.
Predicted Molecular Mass
19.4
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Folkman, J. et al. (2008) FASEB J. 22: 3728
2. Goodsell, DS. et al. (2002) The Oncologist 7: 569
3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130
4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549
5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488
6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.