Recombinant Rat CNTF Rα

Ciliary neurotrophic factor receptor alpha (CNTF Rα) is anchored to the cell membrane by a glycosyl-phosphatidylinositol linkage and is expressed exclusively in the nervous system and skeletal muscle. One possible function of this type of linkage is to allow for the regulated release of this receptor component. Potential physiological roles for the soluble CNTF receptor alpha subunit are suggested by its presence in cerebrospinal fluid and by its release from skeletal muscle in response to peripheral nerve injury.^1,2,3,4

The biological activity of Rat CNTF sRα was determined by its ability to enhance CNTF-dependent TF-1 cell proliferation (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323). The expected ED₅₀ for this effect is typically 0.05 - 0.15 μg/ml in the presence of 10 ng/ml of rrCNTF.

av ytqkhspqea phvqyerlgt dvtlpcgtas wdaavtwrvn gtdlapdlln gsqlilrsle lghsglyacf hrdswhlrhq vllhvglppr epvlscrsnt ypkgfycswh lsaptyipnt fnvtvlhgsk mmvcekdpal knrchirymh lfstikykvs isvsnalghn ttaitfdeft ivkpdppenv varpvpsnpr rlevtwqtps twpdpesfpl kfflryrpli ldqwqhvels ngtahtitda yagkeyiiqv aakdneigtw sdwsvaahat pwteeprhlt teaqapettt sttsslappp ttkicdpgel ssgggp & qkhspqea phvqyerlgt dvtlpcgtas wdaavtwrvn gtdlapdlln gsqlilrsle lghsglyacf hrdswhlrhq vllhvglppr epvlscrsnt ypkgfycswh lsaptyipnt fnvtvlhgsk mmvcekdpal knrchirymh lfstikykvs isvsnalghn ttaitfdeft ivkpdppenv varpvpsnpr rlevtwqtps twpdpesfpl kfflryrpli ldqwqhvels ngtahtitda yagkeyiiqv aakdneigtw sdwsvaahat pwteeprhlt teaqapettt sttsslappp ttkicdpgel ssgggp

Ala19 & Gln23

Lyophilized

The predicted molecular weight of Recombinant Rat CNTF Rα is Mr 39.1 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 52 kDa.

39.1

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Davis, S. et al. (1991)Science 253: 59
2. Davis, S. et al. (1993)Science 259: 1736
3. Davis, S. et al. (1993)Science 260: 1805
4. Ip, NY. et al. (1992)Cell 69: 1121