Anti-Human CNTF Rα

Pricing & Details

Product No.C1396
Clone
121723
Protein
CNTF R alpha
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Ciliary Neurotrophic Factor Receptor Alpha
Isotype
Mouse
IgG2b
Applications
IHC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1396-25 µg
25 µg
$165.00
In stock
Max:
Min: 1
Step: 1
C1396-500 µg
500 µg
$525.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CNTF Rα (Accession # P26992)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CNTF Rα this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CNTF Rα is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C387.
Other Applications Reported In Literature ?
Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human ciliary neurotrophic factor receptor alpha (CNTF Rα) (Clone 121723) recognizes an epitope on Human CNTF Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Ciliary neurotrophic factor receptor alpha (CNTF Rα) is anchored to the cell membrane by a glycosyl-phosphatidylinositol linkage and is expressed exclusively in the nervous system and skeletal muscle. One possible function of this type of linkage is to allow for the regulated release of this receptor component. Potential physiological roles for the soluble CNTF receptor alpha subunit are suggested by its presence in cerebrospinal fluid and by its release from skeletal muscle in response to peripheral nerve injury.1,2,3,4

Antigen Details

References & Citations

1. Davis, S. et al. (1991)Science 253: 59
2. Davis, S. et al. (1993)Science 259: 1736
3. Davis, S. et al. (1993)Science 260: 1805
4. Ip, NY. et al. (1992)Cell 69: 1121
IHC
General Western Blot Protocol
Prod No.
Description
C1396
C1345

Formats Available

Prod No.
Description
C1396
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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