Anti-Human CNTF Rα
Anti-Human CNTF Rα
Product No.: C1396
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Clone 121723 Target CNTF R alpha Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Ciliary Neurotrophic Factor Receptor Alpha Isotype IgG2b Applications IHC , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CNTF Rα (Accession # P26992) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828453 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human CNTF Rα this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CNTF Rα is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C387. Additional Applications Reported In Literature ? Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human ciliary neurotrophic factor receptor alpha (CNTF Rα) (Clone 121723) recognizes an epitope on Human CNTF Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Ciliary neurotrophic factor receptor alpha (CNTF Rα) is anchored to the cell membrane by a glycosyl-phosphatidylinositol linkage and is expressed exclusively in the nervous system and skeletal muscle. One possible function of this type of linkage is to allow for the regulated release of this receptor component. Potential physiological roles for the soluble CNTF receptor alpha subunit are suggested by its presence in cerebrospinal fluid and by its release from skeletal muscle in response to peripheral nerve injury.1,2,3,4 Antigen DetailsPubMed References & Citations1. Davis, S. et al. (1991)Science 253: 59 2. Davis, S. et al. (1993)Science 259: 1736 3. Davis, S. et al. (1993)Science 260: 1805 4. Ip, NY. et al. (1992)Cell 69: 1121 Technical Protocols  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
