Recombinant Rat CXCL7

CXC chemokine ligand 7, also known as CXCL7 and NAP-2, is a cytokine belonging to the CXC chemokine family. It is produced in leukocytes by enzymatic processing of a precursor called platelet basic protein, PBP (1). Mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator are stimulated by CXCL7 (2-3). In addition, CXCL7 chemoattracts and activates neutrophils.

The biological activity of Rat TCK-1 was determined by its ability to chemoattract hCXCR2 transfected mouse BaF/3 cells. The expected ED₅₀ for this effect is typically 1 - 5 ng/mL.

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Ile46

Lyophilized

The predicted molecular weight of Recombinant Rat TCK-1 is Mr 6.8 kDa.

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Poncz, M. et al. (1991) J. Biol. Chem. 266:5785
2. Walz, D. et al. (1983) Proc. Natl. Acad. Sci. USA 80:765
3. Carter-Su, C. et al. (1985) Biochemistry 24:1762