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Chemokine (C-X-C Motif) Ligand 7, PPBP, PBP, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, LA-PF4, LDGF, MDGF, Neutrophil Activating Peptide 2 (NAP-2), SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1
E. coli-Derived Recombinant Mouse CXCL7
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CXCL7 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL7 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2 - 5 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Mouse CXCL7 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL7 (at 40 ng/ml) is 0.8 - 4 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Mouse CXCL7 recognizes Mouse CXCL7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
CXC chemokine ligand 7 is a small cytokine belonging to the CXC chemokine family. It is a protein that is released in large amounts from platelets following their activation.1 It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.2,3
References & Citations
1. Poncz, M. et al. (1991) J Biol Chem. 266: 5785
2. Walz, D. et al. (1983) Proc Natl Acad Sci USA 80: 765
3. Carter-Su, C. et al. (1985) Biochemistry 24: 1762
Products are for research use only. Not for use in diagnostic or therapeutic procedures.