Anti-Mouse CXCL7 (Clone 159742)

Anti-Mouse CXCL7 (Clone 159742)

Product No.: C1380

[product_table name="All Top" skus="C1380"]

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Clone
159742
Target
CXCL7
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
PPBP, PBP, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, LA-PF4, LDGF, MDGF, Neutrophil Activating Peptide 2 (NAP-2), SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1, Thymus Chemokine-1, Chemokine (C-X-C Motif) Ligand 7
Isotype
IgG2b
Applications
N
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse CXCL7 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CXCL7 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL7 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T380.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse CXCL7 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL7 (at 40 ng/ml) is 2-8 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Rat Anti-Mouse CXCL7 (Clone 159742) recognizes an epitope on Mouse CXCL7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. In direct ELISAs, this antibody shows no cross-reactivity with other chemokines tested.
Background
CXC chemokine ligand 7 is a small cytokine belonging to the CXC chemokine family. It is a protein that is released in large amounts from platelets following their activation.1 It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.2,3
PubMed
Research Area
Chemokine

References & Citations

1. Poncz, M. et al. (1991) J Biol Chem. 266: 5785
2. Walz, D. et al. (1983) Proc Natl Acad Sci USA 80: 765
3. Carter-Su, C. et al. (1985) Biochemistry 24: 1762
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General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
C1380
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.