Anti-Mouse CXCL7 (Clone 159742)

Product No.: C1380


Clone 159742 Target CXCL7 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names PPBP, PBP, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, LA-PF4, LDGF, MDGF, Neutrophil Activating Peptide 2 (NAP-2), SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1, Thymus Chemokine-1, Chemokine (C-X-C Motif) Ligand 7 Isotype IgG2b Applications N , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse CXCL7 (>98%) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828436 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse CXCL7 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL7 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T380. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse CXCL7 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CXCL7 (at 40 ng/ml) is 2-8 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rat Anti-Mouse CXCL7 (Clone 159742) recognizes an epitope on Mouse CXCL7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. In direct ELISAs, this antibody shows no cross-reactivity with other chemokines tested. Background CXC chemokine ligand 7 is a small cytokine belonging to the CXC chemokine family. It is a protein that is released in large amounts from platelets following their activation.¹ It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.^2,3 PubMed CXCL7 Research Area Chemokine References & Citations 1. Poncz, M. et al. (1991) J Biol Chem. 266: 5785 2. Walz, D. et al. (1983) Proc Natl Acad Sci USA 80: 765 3. Carter-Su, C. et al. (1985) Biochemistry 24: 1762 View product citations for antibody C1380 on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse CXCL7 (>98%)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828436

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse CXCL7 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL7 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T380.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Mouse CXCL7 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CXCL7 (at 40 ng/ml) is 2-8 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CXCL7 (Clone 159742) recognizes an epitope on Mouse CXCL7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. In direct ELISAs, this antibody shows no cross-reactivity with other chemokines tested.

Background

CXC chemokine ligand 7 is a small cytokine belonging to the CXC chemokine family. It is a protein that is released in large amounts from platelets following their activation.¹ It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.^2,3

PubMed

CXCL7

Research Area

Chemokine

References & Citations

1. Poncz, M. et al. (1991) J Biol Chem. 266: 5785
2. Walz, D. et al. (1983) Proc Natl Acad Sci USA 80: 765
3. Carter-Su, C. et al. (1985) Biochemistry 24: 1762

View product citations for antibody C1380 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
N139	Anti-Human CXCL7 (Clone 59418)
C1380	Anti-Mouse CXCL7 (Clone 159742)
C1478	Anti-Mouse CXCL7
T381	Recombinant Rat CXCL7
T530	Recombinant Mouse Thymus Chemokine-1
C1523	Anti-Mouse CXCL7 – Biotin
N124	Anti-Human CXCL7-Biotin

Formats Available

Prod No.	Description
C1380	Anti-Mouse CXCL7 (Clone 159742)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Mouse CXCL7 (Clone 159742)