Anti-Human CXCL7 (Clone 59418)
Pricing & Details
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Chemokine (C-X-C Motif) Ligand 7, PPBP, PBP, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, LA-PF4, LDGF, MDGF, Neutrophil Activating Peptide 2 (NAP-2), SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1
Purified Recombinant Human CXCL7 (Accession # P02775)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:N124 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:N119.
Western Blotting: To detect Human CXCL7 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL7 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:N119.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human NAP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human NAP-2 (at 1 ng/ml) is 15 - 20 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Chemokine (C-X-C Motif) Ligand 7 (CXCL7) (Clone 59418) recognizes an epitope on Human CXCL7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
CXC chemokine ligand 7 is a small cytokine belonging to the CXC chemokine family. It is a protein that is released in large amounts from platelets following their activation.1 It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.2,3
References & Citations
1. Poncz, M. et al. (1991) J Biol Chem. 266: 5785
2. Walz, D. et al. (1983) Proc Natl Acad Sci USA 80: 765
3. Carter-Su, C. et al. (1985) Biochemistry 24: 1762
Products are for research use only. Not for use in diagnostic or therapeutic procedures.