Recombinant Rat IL-3

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Pricing & Details

Product. No.I-281
Alternate Names
Interleukin-3, Mast Cell Growth Factor [MCGF], Multi-CSF, HCGF, P-Cell Stimulation Factor
Expression Host
E. coli
Species
Rat
Prod No.
Size
Price
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I-281-25 µg
25 µg
$325.00
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Background

IL-3 is a 17 kD pleiotropic cytokine and a member of a family of growth factors that sustains both the proliferation and the development of hematopoietic precursors. It also displays neurotrophic activity and is thought to be associated with neurologic disorders. Signal transmission is assured by high affinity binding to the IL-3 receptor (IL3RA). The receptor contains α and β subunits. IL-3 shares the β subunit with IL-5 and GM-CSF which explains their biological functional similarities. The IL-3/receptor complex induces JAK2/STAT5 cell signalization pathway and it can stimulate both the activation of gene expression and the suppression of apoptosis via transcription factor c‑myc and the Ras pathway, respectively. IL-3 fuels the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or lymphoid progenitor cells (when in conjunction with IL-7). Together with other cytokines, including EPO, GM-CSF, and IL-6, IL-3 can stimulate proliferation of all cells in the myeloid lineage. The constitutive expression of IL-3 in some leukemia cell lines (as opposed to selective T-cell expression in response to specific impulses or antigens) is thought to fuel the development of the disease. Post-chemotherapy IL-3 application can promote regeneration of granulocytes and platelets. Furthermore, during high-dose chemotherapy, the combination of IL-3, GM-CSF and stem cell factor increases peripheral blood stem cells. IL-3 is thought to have therapeutic potential in lymphohematopoietic disorders and solid cancers.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
misdr gsdahhllrt ldcrtialei lvklpypqvs glnnsddkan lrnstlrrvn ldeflksqee fdsqdttdik sklqklkcci paaasdsvlp gvynkdlddf kkklrfyvih lkdlqpvsvs rppqptsssd nfrpmtvec
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Rat IL-3 Beta is Mr 16.3 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed

References & Citations

1. Laston, D. et al. (1998) Science 241:1820
2. Whetton, AD. et al. (1988) Lymphokines 15:355
3. Coleman, JW. et al. (1993) J. Immunol. 150:556
4. Appel, K. et al. (1995) J. Neurosci. 15:5800
5. Orazi, A. et al. (1992) Blood 79:2610
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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