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Anti-Human CD3 (CD3) PerCp/Cy5.5

Monoclonal Antibody

Prod. No.:C2134
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:25 tests, 100 tests
Starting at:$150.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Mouse Anti-Human CD3 (Clone UCHT-1) recognizes Human CD3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein.1 The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of CD3/TCR.2<

Antigen Distribution:
The human CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL cells.

Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias.

Host Species
This PerCp/Cy5.5 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This PerCp/Cy5.5 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
1. Meuer, S. C. et al. (1983) Nature 303:808
2. van Dongen, J. J. M. et al. (1988) Blood 71:603
3. Beverly, P. C. L. et al. (1981) Eur. J. Immunol. 11:329
4. Leukocyte Typing: 3rd Workshop: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
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