Product Datasheet
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Anti-Mouse CD3ε (CD3ε) Biotin

(CD3, T3)
Monoclonal Antibody


Prod. No.:C2235
Clone:145-2C11
Isotype:Armenian Hamster IgG
Conc.:0.5 mg/ml
Pkg. Size:100 ug, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Armenian Hamster Anti-Mouse CD3ε (CD3ε) (Clone 145-2C11) recognizes an epitope on Mouse CD3ε. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Known Species Cross Reactivity: Ms+
Host Species
Armenian Hamster
Immunogen
Purified Recombinant Mouse CD3ε (>98%)
Formulation
This Biotin conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3ε. A suggested method would be to stain cells expressing CD3ε with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104)
Anti-Mouse CD3ε-Biotin (Leinco Prod. No.: C208)
PN:
Anti-Mouse CD3ε Purified (Leinco Prod. No.: C207)
Anti-Mouse CD3ε Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C219)
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