Anti-β 3 GABA-A Receptor [Clone S87-25]
Anti-β 3 GABA-A Receptor [Clone S87-25]
Product No.: 56482
Data
Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25 (56482). Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (56482) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25 (56482). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (56482) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody, Clone N87/25 (56482). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody (56482) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Beta 3 Antibody (D) Composite. - -
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein corresponding to aa 370-433 of mouse Beta 3 GABA-A receptor (accession no. AAB60502). Product Concentration Lot Specific Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-10ug/mL. A band of ~55kDa is detected.
Immunohistochemistry: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human, mouse, and rat Beta 3 GABA-A receptor. It does not cross-react with Beta 1 or 2 GABA-A receptor. Background Ion channels are integral membrane proteins thathelp establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. In some ion channels, the flow of ions is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. The GABA-A receptor is a fast acting ligand-gated ion channel. GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain. Function Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:9108119). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA- gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta3/gamma2 receptor exhibits synatogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (PubMed:27129275). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). Plays an important role in somatosensation and in the production of antinociception (PubMed:10670447). {UniProtKB:P28472, PubMed:10670447, PubMed:27129275, PubMed:9108119}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical ProtocolsICC IF   AM Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
