Anti-6 His Antibody, HRP Conjugate

Anti-6 His Antibody, HRP Conjugate

Product No.: 18814P

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Target
6 His
Product Type
Polyclonal
Isotype
Whole IgG
Applications
ELISA
,
ICC
,
IHC
,
WB

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Select Product Size

Data

<b>Detection of 6-His-tagged Protein by western blot.</b> <i>Samples:</i> 200Detection of 6-His tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18814P diluted 1:25,000.
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Antibody Details

Product Details

Host Species
Rabbit
Immunogen
6-His (HHHHHH) conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400. Molar enzyme/antibody ratio is 4:1.
State of Matter
Liquid
Product Preparation
Purified by peptide immuno-affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at 2-8°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at a dilution of 1:1,000-1:30,000

ELISA: use at a dilution of 1:10,000-1:100,000.

Immunocytochemistry: use at a dilution of 1:200-1:500

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Background
A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Antigen Details

Research Area
Epitope Tags

References & Citations

Indirect Elisa Protocol
ICC
IHC
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.