Anti-Ankyrin-B Antibody (56518)
Anti-Ankyrin-B Antibody (56518)
Product No.: 56518
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin B Monoclonal Antibody, Clone S105-13 (56518). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody (56518) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin B Antibody (D) Composite.
Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin B Antibody. (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin B Monoclonal Antibody, Clone S105-13 (56518). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody (56518) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm - -
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Synthetic peptide correspond- ing to aa 203-496 of human Ankyrin-B (accession no. NP_001120965.1) Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for
use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-2ug/mL. A band of >200kDa is detected.
Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human, mouse,
and rat Ankyrin-B. Background Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and for at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers, and ion transporters in the plasma membrane. Function Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:12571597). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (By similarity). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (By similarity). {UniProtKB:Q8C8R3, PubMed:12571597}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical ProtocolsICC IF   Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
