Data
Detection of human ATR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATR antibody (13016 lot 3) used for IP at 6 µg per reaction. ATR was also immunoprecipitated by a previous lot of this antibody (13016 lot 2) and a second antibody against a different epitope of rabbit ATR (13018). For blotting immunoprecipitated ATR|13016 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human ATR by western blot. Samples: Whole cell lysate (10 µg) from U2OS, HeLa, MCF-7, RKO, and My-La CD4+ cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody (13016 lot 3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse Host Species Rabbit Immunogen Synthetic peptide representing a portion of the protein encoded within exon 5 (LocusLink ID 545). Product Concentration Lot Specific Formulation Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide State of Matter Liquid Product Preparation Purified by immunoaffinity chromatography Storage and Handling Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Western Blot: 0.033 - 0.067 ug/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit polyclonal antibody specific to ATR of human and mouse. Antigen DetailsFunction Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. RUniProtKB:Q9JKK8, PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11114888, PubMed:11418864, PubMed:11673449, PubMed:11721054, PubMed:11865061, PubMed:12526805, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15314022, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:27723717, PubMed:27723720, PubMed:33848395, PubMed:9427750, PubMed:9636169, PubMed:9925639}. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsTechnical Protocols  |
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