Anti-c-myc Antibody, HRP conjugate (18826P)

Anti-c-myc Antibody, HRP conjugate (18826P)

Product No.: 18826P

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Target
c-Myc
Product Type
Polyclonal
Alternate Names
Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64
Isotype
Whole IgG
Applications
ELISA
,
ICC
,
IHC
,
WB

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Select Product Size

Data

<b>Detection of c-myc-tagged Protein by western blot.</b> <i>Samples:</i> 200Detection of c-myc-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-Myc Tag antibody 18826P used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 10 seconds.
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Antibody Details

Product Details

Host Species
Rabbit
Immunogen
Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
State of Matter
Liquid
Product Preparation
Purified by peptide immuno-affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at 2-8°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at a dilution of 1:1,000- 1:30,000.

Immunocytochemistry: use at a dilution of 1:200-1:500

ELISA: use at a dilution of 1:10,000- 1:100,000

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to c-myc.
Background
c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids.

Antigen Details

Function
Transcription factor that binds DNA in a non-specific manner, yet also specifically rUniProtKB:P01108, PubMed:20010808, PubMed:24940000, PubMed:25956029}.
NCBI Gene Bank ID
UniProt.org
Research Area
Epitope Tags

References & Citations

Indirect Elisa Protocol
ICC
IHC
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.