Data
Detection of human CDC25a by western blot of immunoprecipitates. Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody 13022 (lot 13022-2) used for IP at 6 µg per reaction. CDC25a was also immunoprecipitated by rabbit anti-CDC25a antibody. For blotting immunoprecipitated CDC25a, 13022 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody 13022 (lot 13022-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. - -
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Antibody DetailsProduct DetailsReactivity Species Human Host Species Rabbit Immunogen Synthetic peptide representing a portion of the protein encoded by exon 7 (LocusLink ID 993). Product Concentration Lot Specific Formulation Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide State of Matter Liquid Product Preparation Purified by immunoaffinity chromatography Storage and Handling Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Western Blot: 0.1 - 1 ug/ml.
Immunoprecipitation: 1- 4 ug/mg of lysate. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit polyclonal antibody specific to human CDC25a, other species have not been tested. Antigen DetailsFunction Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsTechnical Protocols  |
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