Anti-Elastase, Neutrophil Antibody

Anti-Elastase, Neutrophil Antibody

Product No.: 13203

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Clone
ELA10-101.5
Target
Elastase, Neutrophil,
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
EC 3.4.21.37, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase
Isotype
Mouse IgG1
Applications
ELISA
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Data

These antibodies recognize human neutrophil elastase and vary in their ability to inhibit neutrophil elastase activity as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate:These antibodies recognize human neutrophil elastase and vary in their ability to inhibit neutrophil elastase activity as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate.
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified human neutrophil elastase.
Product Concentration
Lot Specific
Formulation
This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added.
State of Matter
Liquid
Product Preparation
Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
These antibodies have been qualified for use in ELISA to detect human neutrophil elastase and in studies of inhibition and enzyme kinetics of human neutrophil elastase.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
This antibodies recognizes human neutrophil elastase and inhibits neutrophil elastase activity by 90% as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate.
Background
Neutrophil elastase is a major neutral protease present in human neutrophils and is released by neutrophils following stimulation with a variety of stimuli. It is believed to be a major mediator of the lung destruction seen in emphysema. In addition to elastin, neutrophil elastase can cleave other biologically active proteins such as fibrinogen and antithrombin and is released by neutrophils during clot formation.
Function
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). {PubMed:10947984, PubMed:15140022}.
NCBI Gene Bank ID
UniProt.org
Research Area
Enzymes

References & Citations

Indirect Elisa Protocol
N

Certificate of Analysis

Formats Available

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Prod No.
Description
13203
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.