Anti-Elastase Neutrophil [Clone ELA10-103.2]
Anti-Elastase Neutrophil [Clone ELA10-103.2]
Product No.: 13204
Data
These antibodies recognize human neutrophil elastase and vary in their ability to inhibit neutrophil elastase activity as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate. - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified human neutrophil elastase. Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco These antibodies have been qualified for use in ELISA to detect human neutrophil elastase and in studies of inhibition and enzyme kinetics of human neutrophil elastase. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibodies recognizes human neutrophil elastase and inhibits neutrophil elastase activity by 82% as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate. Background Neutrophil elastase is a major neutral protease present in human neutrophils and is released by neutrophils following stimulation with a variety of stimuli. It is believed to be a major mediator of the lung destruction seen in emphysema. In addition to elastin, neutrophil elastase can cleave other biologically active proteins such as fibrinogen and antithrombin and is released by neutrophils during clot formation. Function Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). {PubMed:10947984, PubMed:15140022}. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsTechnical Protocols N Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
