Data
Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10 (11574). Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody (11574) at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.
Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10 (11574). Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody (11574) at 1:1000. Predicted/Observed Size: ~85kDa. - -
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Antibody DetailsProduct DetailsReactivity Species Mouse ⋅ Rat Host Species Mouse Immunogen Synthetic peptide corresponding to N-terminal aa 46-68 of rat ENaC alpha. Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Store product in appropriate aliquots to
avoid multiple freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL.
Immunofluorescence:Detection of ~85kDa ENaC alpha with #11574 at 1ug/mL.Detection of ENaC alpha (green) in paraffin- embedded, formalin-fixed rat kidney with #11574 at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse and rat ENaC alpha Background The epithelial sodium channel (ENaC) is a membrane-bound ion-channel that is selectively permeable to Na+ ions and that is assembled as a heterotrimer composed of three homologous subunits alpha, beta, and g. It is involved primarily in the reabsorption of sodium ions in the collecting ducts of the kidney's nephrons. These channels mediate the first step of active sodium reabsorption essential for the maintenance of body salt and water homeostasis. In vertebrates, the channels control reabsorption of sodium in kidney, colon, lung and sweat glands; they also play a role in taste perception. Antigen DetailsNCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical Protocols  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
