Anti-Entamoeba Histolytica [Clone EH147.5] [IgM Isotype]
Anti-Entamoeba Histolytica [Clone EH147.5] [IgM Isotype]
Product No.: 15010
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Clone EH147.5 Target Entamoeba histolytica Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names E. histolytica Isotype Mouse IgM Applications ELISA |
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Antibody DetailsProduct DetailsReactive Species E. histolytica Host Species Mouse Immunogen Entamoeba histolytica HK-9 Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Preparing IgM antibodies presents a unique set of challenges compared to standard IgG molecules. Because IgMs are massive pentameric structures (approximately 900 kDa), they are highly sensitive to shear stress, precipitation, and aggregation. Leinco overcomes these biochemical hurdles by combining advanced in vitro upstream production with a specialized downstream purification pipeline at our ISO 13485 certified facility in St. Louis, Missouri USA. Storage and Handling This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco This antibody has been qualified for use in ELISA to detect E. histolytica trophozoites and cysts. End users should determine optimal concentrations for their applications. All IgM isotype clones undergo strict analytical validation to ensure it meets our rigorous product specification (PSDs) for diagnostic raw materials or research use specifications. Every lot is verified for: - Structural Integrity: Analyzed via SDS-PAGE and FPLC to confirm pentameric purity. - Ultra-Clean Formulation: Delivered in PBS (pH 7.2–7.4) entirely carrier-free (no BSA or gelatin) and preservative-free (no sodium azide), ensuring it is instantly ready for custom conjugation or assay integration. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity These antibodies recognize Entamoeba histolytica trophozoites and cysts. Background Entamoeba histolytica is an anaerobic parasitic protozoan that predominantly infects humans and other primates causing amoebiasis. In the vast majority of cases, infection is asymptomatic and the carrier is unaware they are infected. However, in an estimated 10% of cases E. histolytica causes disease. Once the trophozoites are excysted they colonize the large bowel, remaining on the surface of the mucus layer and feeding on bacteria and food particles. Occasionally, and in response to unknown stimuli, trophozoites move through the mucus layer where they come in contact with the epithelial cell layer and start the pathological process. Research Area Infectious Disease References & CitationsTechnical Protocols Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
