Anti-Estrogen Receptor β [Clone 6A12]
Anti-Estrogen Receptor β [Clone 6A12]
Product No.: 3336
Data
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12] (3336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-estrogen-receptor-beta-antibody-3336-1.jpg)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12] (3336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen 6-His fusion protein containing the region encoding aa 1-153 of human estrogen receptor-b (ER-b) expressed in E. coli. Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-5 ug/mL.
Positive controls: Native receptor from human thymus, spleen, ovary, and testis or recombinant protein. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human ER-b, a novel receptor (approx. 52 kD) recently isolated from testis. The size and structure of ER-b are very similar to ER-alpha with the ligand and DNA binding domains being highly conserved. The N-terminus of each receptor, which serves as their transactivation domain, are significantly different. Like ER-alpha, ER-b binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements. Function Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). {PubMed:20074560, PubMed:29261182, PubMed:30113650, PubMed:9325313}.; [Isoform 2]: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. {PubMed:9671811}. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & CitationsTechnical ProtocolsICC IF   Chromatin IP (ChIP) Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
