Anti-Estrogen Receptor β Antibody (3336)

Product No.: 3336


Clone 6A12 Target Estrogen Receptorα Formats AvailableView All Product Type Monoclonal Alternate Names ER-β, Nuclear receptor subfamily 3 group A member 2 Isotype Mouse IgG2b Applications WB,ICC/IF,ChIP,IHC


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Data

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12] (3336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen 6-His fusion protein containing the region encoding aa 1-153 of human estrogen receptor-b (ER-b) expressed in E. coli. Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-5 ug/mL. Positive controls: Native receptor from human thymus, spleen, ovary, and testis or recombinant protein. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity This antibody recognizes human ER-b, a novel receptor (approx. 52 kD) recently isolated from testis. The size and structure of ER-b are very similar to ER-alpha with the ligand and DNA binding domains being highly conserved. The N-terminus of each receptor, which serves as their transactivation domain, are significantly different. Like ER-alpha, ER-b binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements. Function Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). {PubMed:20074560, PubMed:29261182, PubMed:30113650, PubMed:9325313}.; [Isoform 2]: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. {PubMed:9671811}. NCBI Gene Bank ID ESR2 UniProt.org Q92731 Research Area Cancer Research References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

6-His fusion protein containing the region encoding aa 1-153 of human estrogen receptor-b (ER-b) expressed in E. coli.

Product Concentration

Lot Specific

Formulation

PBS, pH 7.4

State of Matter

Liquid

Product Preparation

Purified by Protein G affinity chromatography

Storage and Handling

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting: use at 1-5 ug/mL.

Positive controls: Native receptor from human thymus, spleen, ovary, and testis or recombinant protein.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes human ER-b, a novel receptor (approx. 52 kD) recently isolated from testis. The size and structure of ER-b are very similar to ER-alpha with the ligand and DNA binding domains being highly conserved. The N-terminus of each receptor, which serves as their transactivation domain, are significantly different. Like ER-alpha, ER-b binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements.

Function

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). {PubMed:20074560, PubMed:29261182, PubMed:30113650, PubMed:9325313}.; [Isoform 2]: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. {PubMed:9671811}.

NCBI Gene Bank ID

ESR2

UniProt.org

Q92731

Research Area

Cancer Research