Anti-GABA Receptor Associated Protein Antibody (56300)

Anti-GABA Receptor Associated Protein Antibody (56300)

Product No.: 56300

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Target
GABA Receptor Associated Protein
Product Type
Polyclonal
Alternate Names
GABA(A receptor-associated protein, MM46
Isotype
Rabbit
Applications
WB,ELISA

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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Peptide derived from the N- terminus of GABARAP.
Product Concentration
Lot Specific
Formulation
PBS, pH 7.4 with 0.02% sodium azide
State of Matter
Liquid
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at 1:500-1:1,000 dilution

ELISA: use at 1:1,000-1:5,000 dilution.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to GABARAP
Background
Type-A receptors for the neurotransmitter GABA (gamma- aminobutyric acid) are ligand-gated chloride channels that mediate inhibitory neurotransmission. A cellular protein, GABA(A)-receptor associated protein (GABARAP), that interacts with the gamma 2 subunit of GABA(A) receptors and modulates receptor channel kinetics was recently identified. GABARAP binds to GABA(A) receptors in vitro and in vivo. Sequence analysis shows similarity between GABARAP and light chain-3 of microtubule-associated proteins 1A and 1B. The interactions between GABA(A) receptors, GABARAP and tubulin suggest a mechanism for the targeting and clustering of GABA(A) receptors.

Antigen Details

Function
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355). Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538). Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205). Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205). Involved in apoptosis (PubMed:15977068). {PubMed:15169837, PubMed:15977068, PubMed:20562859, PubMed:22948227, PubMed:25684205, PubMed:31006538, PubMed:9892355}.
NCBI Gene Bank ID
UniProt.org
Research Area
Neuroscience

References & Citations

WB,ELISA
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.