Anti-Hsp90 [Clone H90-10]
Anti-Hsp90 [Clone H90-10]
Product No.: 11098
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Clone H90-10 Target HSP 90-β Formats AvailableView All Product Type Monoclonal Alternate Names HSP 90, Heat shock 84 kDa, HSP 84, HSP84 Isotype Mouse IgG2a Applications ELISA , ICC , IF , IHC , IP , WB , AM |
Data
Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (11098). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (11098) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.
Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (11098). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (11098) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.
Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (11098). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (11098) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (11098). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (11098) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rabbit Host Species Mouse Immunogen Recombinant human Hsp90β (Accession NP_031381.2) Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-5ug/mL. A band of ~90kDa is detected. Detection of Hsp90β in tumor cell lines and rat brain with #11098 at 1ug/mL. Detection of Hsp90β in HeLa cell lysate with #11098 at 1ug/mL.
Immunohistochemistry: use at 0.5-10ug/mL. Detection of Hsp90β in human colon carcinoma Detection of Hsp90β in mouse skin with #11098 with #11098 at 0.5ug/mL. at 10ug/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Detects 90kDa Hsp90β in all reactive species except chicken where it detects both
alpha and beta isoforms. Background Hsp90 and Grp94: Essential Molecular Chaperones Hsp90 and the 94 kDa glucose-regulated protein (Grp94) are key molecular chaperones found in the cytosol and endoplasmic reticulum, respectively. In mammalian cells, there are at least two distinct isoforms of Hsp90, Hsp90α and Hsp90β, each encoded by a separate gene. All known members of the Hsp90 family are remarkably conserved, particularly in their N-terminal and C-terminal regions. Even without cellular stress, Hsp90 plays a critical role in essential cellular processes like hormone signaling and cell cycle control. Many important regulatory proteins, including steroid receptors, cell cycle kinases, and p53, have been identified as substrates that rely on Hsp90 for proper folding and function. Antigen DetailsFunction Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate rPubMed:16478993, PubMed:18239673, PubMed:19696785, PubMed:20353823, PubMed:24613385, PubMed:32272059, PubMed:25973397, PubMed:26991466, PubMed:27295069}. NCBI Gene Bank ID UniProt.org Research Area Heat Shock & Stress Proteins References & Citations1. Barent RL et al. 1998 Mol Endocrinol 12: 342. 2. Felts SJ et al. 2000 J Biol Chem 275: 3305. 3. Arlander SJ et al. 2003 J Biol Chem 278: 52572. 4. Wang, Q., Li, X., et al.Stem Cell Res Ther. 2019. 10(1):348 5. Lei, W., Duron, D. I., et al.Front Mol Neurosci. 2019. 12:294 Technical Protocols ICC IF    AM |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
