Anti-Human 4-1BBL -Biotin (CD137L)

Pricing & Details

Product No.L248
Protein
4-1BBL
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
TNFSF9, CD137L, 4-1BBL
Applications
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
L248-50 µg
50 µg
$419.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human 4-1BB Ligand (aa 71-254) (Accession # P41273)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human 4-1BB Ligand this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human 4-1BB Ligand is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F131.
Flow Cytometry: Suitable for use at concentration 2.5 µg/106 cells
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human 4-1BB Ligand recognizes Human 4-1BB Ligand. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Flow Cytometry
General Western Blot Protocol
Prod No.
Description
L248
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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