Anti-Human CD137L (4-1BB)

Pricing & Details

Product No.L251
Clone
282220
Protein
CD137L
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNFSF9, 4-1BB Ligand, 4-1BBL
Isotype
Mouse
IgG2b
Applications
CyTOF®
,
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
L251-25 µg
25 µg
$175.00
In stock
Max:
Min: 1
Step: 1
L251-100 µg
100 µg
$339.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human 4-1BB Ligand (>98%) (Accession # P41273.1)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing 4-1BB Ligand. A suggested method would be to stain cells expressing 4-1BB Ligand with 2.5 µg/106 cells in a total staining volume of 200 µl followed by PN:M1189.
Western Blotting: To detect Human 4-1BB Ligand this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human 4-1BB Ligand is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F131.
Other Applications Reported In Literature ?
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human 4-1BB Ligand (Clone 282220) recognizes an epitope on Human 4-1BB Ligand. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

Protein
Ligand/Receptor
4-1BB (CDw137)
PubMed
NCBI Gene Bank ID
Flow Cytometry
General Western Blot Protocol

Formats Available

Prod No.
Description
L251
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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