Purified Recombinant Human AITRL/TNFSF18 (>98%)
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells
Other Applications Reported In Literature ?
Blocking: In a functional ELISA, <5 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Human GITR Ligand/TNFSF18 to immobilized Recombinant Human GITR/TNFRSF18 Fc Chimera coated at 2 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human AITRL (TNFSF18) recognizes Human TNFSF18. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Tumor necrosis factor (ligand) superfamily, member 18, also known as TNFSF18 and AITRL, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells.
NCBI Gene Bank ID
Products are for research use only. Not for use in diagnostic or therapeutic procedures.