Antibody DetailsProduct DetailsReactivity Species Human Host Species Mouse Immunogen Purified Recombinant Human ApoE (Accession # P02649) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828158 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human ApoE this monoclonal antibody can be used at a concentration of 0.5 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human ApoE is 10 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human ApoE (Clone 395004) recognizes an epitope on Human ApoE. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Sennlaub, F. et al. (2015) J Neurosci. 35(40): 13568–13576. PubMed Technical Protocols |
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