Pricing & Details


Product No.B643 Protein BAK Formats AvailableView All Product Type Polyclonal Antibody Applications IHC , WB Submit A Review	Printable Datasheet Printable SDS

Prod No.	Size	Price	Avail.	Qty	Add to cart
B643-25 µg	25 µg	$205.00	In stock	Max: Min: 1 Step: 1	Add to Cart
B643-100 µg	100 µg	$375.00	In stock	Max: Min: 1 Step: 1	Add to Cart

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Rabbit

Immunogen

Purified Recombinant Human BAK (aa 53-72)(>98%)

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828300

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human BAK this polyclonal antibody can be used at a concentration of 1.0 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R1190 and a chromogenic substrate such as PN:T343. The detection limit for Human BAK is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Other Applications Reported In Literature ?

Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Anti-Human (BAK) recognizes Human BAK. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.