Anti-Human Bcl-2 (Intermediate Domain)

PN:B471

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human Bcl-2 recognizes an epitope in the intermediate domain of Human Bcl-2. This polyclonal antibody was purified using affinity chromatography.

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer.² Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis.^2,3 These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis.⁴ It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad.⁵ Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.^4,6

1. Lockshin, RA. et al. (2000) Cell Death Differ. 7:2-7.
2. Cory, S. et al. (2003) Oncogene 22:8590-607.
3. Heiser, D. et al. (2004) Exp. Geron. 39:1125-35.
4. Hockenbery, D. et al. (1990) Nature 348:334-6.