This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human Bcl-2 recognizes an epitope near the N-terminus of human and mouse Bcl-2. This polyclonal antibody was purified using affinity chromatography.
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer.2 Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis.2,3 These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis.4 It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad.5 Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.4,6
References & Citations
1. Lockshin, RA. et al. (2000) Cell Death Differ. 7:2-7.
2. Cory, S. et al. (2003) Oncogene 22:8590-607.
3. Heiser, D. et al. (2004) Exp. Geron. 39:1125-35.
4. Hockenbery, D. et al. (1990) Nature 348:334-6.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.